Complex repeat structure promotes hyper-amplification and amplicon evolution through rolling-circle replication.

ABSTRACT: Inverted repeats (IRs) are abundant in genomes and frequently serve as substrates for chromosomal aberrations, including gene amplification. In the early stage of amplification, repeated cycles of chromosome breakage and rearrangement, called breakage-fusion-bridge (BFB), generate a large inverted structure, which evolves into highly-amplified, complex end products. However, it remains to be determined how IRs mediate chromosome rearrangements and promote subsequent hyper-amplification and amplicon evolutions. To dissect the complex processes, we constructed repetitive structures in a yeast chromosome and selected amplified cells using genetic markers with limited expression. The genomic architecture was associated with replication stress and produced extra-/intra-chromosomal amplification

SUBMITTER: Watanabe T 

PROVIDER: S-EPMC6007334 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature