Analysis of ParAB dynamics in mycobacteria shows active movement of ParB and differential inheritance of ParA.

ABSTRACT: Correct chromosomal segregation, coordinated with cell division, is crucial for bacterial survival, but despite extensive studies, the mechanisms underlying this remain incompletely understood in mycobacteria. We report a detailed investigation of the dynamic interactions between ParA and ParB partitioning proteins in Mycobacterium smegmatis using microfluidics and time-lapse fluorescence microscopy to observe both proteins simultaneously. During growth and division, ParB presents as a focused fluorescent spot that subsequently splits in two. One focus moves towards a higher concentration of ParA at the new pole, while the other moves towards the old pole. We show ParB movement is in part an active process that does not rely on passive movement associated with cell growth. In some cells, a

SUBMITTER: Uhia I 

PROVIDER: S-EPMC6007833 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature