Defining the rhesus macaque placental miRNAome: Conservation of expression of placental miRNA clusters between the macaque and human.

ABSTRACT: OBJECTIVE:Expression of microRNAs (miRNAs) in the human placenta is dynamic across gestation, with expression of miRNAs belonging to the C14MC, C19MC and miR-371-3 clusters. Specifically, miRNAs within the C19MC cluster are exclusively expressed in primates with predominant expression in the placenta. Non-human primates can be utilized to study developmental processes of placentation in vivo that cannot be assessed in the human placenta, however, miRNA expression has not been defined in the macaque placenta. Our objective was to profile miRNAs in the macaque placenta, hypothesizing that expression is conserved between the macaque and human placenta. METHODS:Total RNA from first trimester and term macaque placentas (n = 4 per group) was analyzed through RNA-sequencing and validated by quant

SUBMITTER: Schmidt JK 

PROVIDER: S-EPMC6007866 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature