Identification of reference genes for RT-qPCR in the Antarctic moss Sanionia uncinata under abiotic stress conditions.

ABSTRACT: Sanionia uncinata is a dominant moss species in the maritime Antarctic. Due to its high adaptability to harsh environments, this extremophile plant has been considered a good target for studying the molecular adaptation mechanisms of plants to a variety of environmental stresses. Despite the importance of S. uncinata as a representative Antarctic plant species for the identification and characterization of genes associated with abiotic stress tolerance, suitable reference genes, which are critical for RT-qPCR analyses, have not yet been identified. In this report, 11 traditionally used and 6 novel candidate reference genes were selected from transcriptome data of S. uncinata and the expression stability of these genes was evaluated under various abiotic stress conditions using three statis

SUBMITTER: Park M 

PROVIDER: S-EPMC6007896 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature