Identification, characterization and application of a new peptide against anterior gradient homolog 2 (AGR2).

ABSTRACT: The cancer-associated protein Anterior Gradient 2 (AGR2) has been described, predominantly in adenocarcinomas. Increased levels of extracellular AGR2 (eAGR2) have been correlated with poor prognosis in cancer patients, making it a potential biomarker. Additionally, neutralizing AGR2 antibodies showed preclinical effectiveness in murine cancer models suggesting eAGR2 may be a therapeutic target. We set out to identify a peptide by mRNA display that would serve as a theranostic tool targeting AGR2. This method enables the selection of peptides from a complex (>10¹¹) library and incorporates a protease incubation step that filters the selection for serum stable peptides. We performed six successive rounds of enrichment using a 10-amino acid mRNA display library and identified sever

SUBMITTER: Garri C 

PROVIDER: S-EPMC6007958 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature