High miR-100 expression is associated with aggressive features and modulates TORC1 complex activation in lung carcinoids.

ABSTRACT:

Purpose

Mammalian target of rapamycin (mTOR) is a promising therapeutic target in advanced lung carcinoid patients. However, the mechanisms of mTOR modulation and of responsiveness to mTOR inhibitors are largely unclear. Our aim was to analyze the expression and functional role of specific miRNAs in lung carcinoids as an alternative mechanism targeting mTOR pathway.

Experimental design

Seven miRNAs, selected by bioinformatic tools and literature search, were analyzed in 142 lung neuroendocrine neoplasms (92 carcinoids and a control group of 50 high grade neuroendocrine carcinomas), and compared with mTOR mRNA expression and clinical/pathological parameters. Tissue results were validated in vitro in two lung carcinoid cell lines by specific RNA interference and biologi

SUBMITTER: Rapa I 

PROVIDER: S-EPMC6007959 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature