Identifying patients with an unfavorable prognosis in early stages of colorectal carcinoma.

ABSTRACT:

Background

In recent years, the concept of liquid biopsy diagnostics in detection and progress monitoring of malignant diseases gained significant awareness. We here report on a semi-quantitative real-time cytokeratin 20 RT-PCR-based assay, for detecting circulating tumor cells within a fraction of peripheral blood mononuclear cells in colorectal cancer patients.

Methods

In total, 381 patients were included. Prior to surgical tumor resection, a peripheral blood sample was drawn. Mononuclear cells were isolated by Ficoll centrifugation and a cytokeratin 20 qRT-PCR assay was performed. Quantitative PCR data was assessed regarding histopathological characteristics and patients´ clinical outcome.

Results

A cut-off value was determined at ≥ 2.77 [EU]. Stratifying patients

SUBMITTER: Hendricks A 

PROVIDER: S-EPMC6007960 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature