Standardization of a protocol for shotgun proteomic analysis of saliva.

ABSTRACT:

Introduction

Saliva contains numerous proteins and peptides, each of them carries a number of biological functions that are very important in maintaining the oral cavity health and also yields information about both local and systemic diseases. Currently, proteomic analysis is the basis for large-scale identification of these proteins and discovery of new biomarkers for distinct diseases.

Objective

This study compared methodologies to extract salivary proteins for proteomic analysis.

Material and methods

Saliva samples were collected from 10 healthy volunteers. In the first test, the necessity for using an albumin and IgG depletion column was evaluated, employing pooled samples from the 10 volunteers. In the second test, the analysis of the pooled samples was compared

SUBMITTER: Ventura TMDS 

PROVIDER: S-EPMC6007968 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature