Apoptosis of enterocytes and nitration of junctional complex proteins promote alcohol-induced gut leakiness and liver injury.

ABSTRACT: BACKGROUND & AIMS:Binge alcohol exposure causes gut leakiness, contributing to increased endotoxemia and inflammatory liver injury, although the molecular mechanisms are still elusive. This study was aimed at investigating the roles of apoptosis of enterocytes and nitration followed by degradation of intestinal tight junction (TJ) and adherens junction (AJ) proteins in binge alcohol-induced gut leakiness. METHODS:The levels of intestinal (ileum) junctional complex proteins, oxidative stress markers and apoptosis-related proteins in rodents, T84 colonic cells and autopsied human ileums were determined by immunoblot, immunoprecipitation, immunofluorescence, and mass-spectral analyses. RESULTS:Binge alcohol exposure caused apoptosis of gut enterocytes with elevated serum endotoxin and liver i

SUBMITTER: Cho YE 

PROVIDER: S-EPMC6008177 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature