Unknown

Dataset Information

0

Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.

ABSTRACT: Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

SUBMITTER: Bosse JT 

PROVIDER: S-EPMC6008488 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.

Bossé Janine T JT   Li Yanwen Y   Fernandez Crespo Roberto R   Lacouture Sonia S   Gottschalk Marcelo M   Sárközi Rita R   Fodor László L   Casas Amoribieta Maria M   Angen Øystein Ø   Nedbalcova Katerina K   Holden Matthew T G MTG   Maskell Duncan J DJ   Tucker Alexander W AW   Wren Brendan W BW   Rycroft Andrew N AN   Langford Paul R PR  

Veterinary microbiology 20180520

Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleurop  ...[more]

Similar Datasets

Unknown Association of Actinobacillus pleuropneumoniae capsular polysaccharide with virulence in pigs.
| S-EPMC155728 | biostudies-literature
Unknown The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 15.
| S-EPMC4427752 | biostudies-literature
Unknown The genetic organization of the capsular polysaccharide biosynthesis region of Actinobacillus pleuropneumoniae serotype 14.
| S-EPMC4478738 | biostudies-literature
Unknown Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis.
| S-EPMC5901230 | biostudies-literature
Unknown Rapid Detection and Typing of <i>Actinobacillus pleuropneumoniae</i> Serovars Directly From Clinical Samples: Combining FTA<sup>®</sup> Card Technology With Multiplex PCR.
| S-EPMC8382971 | biostudies-literature
Unknown <i>In silico</i> capsule locus typing for serovar prediction of <i>Actinobacillus pleuropneumoniae</i>.
| S-EPMC9453067 | biostudies-literature
Unknown Genetic Diversity of <i>Actinobacillus pleuropneumoniae</i> Serovars in Hungary.
| S-EPMC9607985 | biostudies-literature
Unknown Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12.
| S-EPMC4097740 | biostudies-literature
Unknown DNA microarray-based identification and typing of Actinobacillus pleuropneumoniae.
| S-EPMC2705073 | biostudies-literature
Unknown Detection and identification of Actinobacillus pleuropneumoniae serotype 5 by multiplex PCR.
| S-EPMC104904 | biostudies-literature