Different Expression Levels of Human Mutant Ubiquitin B⁺¹ (UBB⁺¹) Can Modify Chronological Lifespan or Stress Resistance of Saccharomyces cerevisiae.

ABSTRACT: The ubiquitin-proteasome system (UPS) is the main pathway responsible for the degradation of misfolded proteins, and its dysregulation has been implicated in several neurodegenerative diseases, including Alzheimer's disease (AD). UBB⁺¹, a mutant variant of ubiquitin B, was found to accumulate in neurons of AD patients and it has been linked to UPS dysfunction and neuronal death. Using the yeast Saccharomyces cerevisiae as a model system, we constitutively expressed UBB⁺¹ to evaluate its effects on proteasome function and cell death, particularly under conditions of chronological aging. We showed that the expression of UBB⁺¹ caused inhibition of the three proteasomal proteolytic activities (caspase-like (β1), trypsin-like (β2) and chymotrypsin-like (β

SUBMITTER: Munoz-Arellano AJ 

PROVIDER: S-EPMC6008557 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature