Gene expression of CCL8 and CXCL10 in peripheral blood leukocytes during early pregnancy in cows.

ABSTRACT:

Background

The aim of the present study was to evaluate CCL8 and CXCL10 expression and its regulatory mechanism in peripheral blood leukocytes (PBLs) at the time of maternal recognition in cows. Blood samples were collected on 14, 15, 16, 17 and 18 d after artificial insemination (AI). Based on the day of return of estrus, cows were divided into three groups, pregnant (n = 5), early embryonic mortality (EEM; n = 5) and late embryonic mortality (LEM; n = 5). The gene expression levels in PBLs were assessed with quantitative real-time reverse transcription PCR.

Results

The expression of CCL8 and CXCL10 mRNA in PBLs gradually increased from 14 to 18 d of pregnant cows and significant differences were observed on 18 d (P < 

SUBMITTER: Sakumoto R 

PROVIDER: S-EPMC6008911 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature