NICD-mediated notch transduction regulates the different fate of chicken primordial germ cells and spermatogonial stem cells.

ABSTRACT:

Background

Notch signaling is mainly regulated by Notch1 during development of chicken germ stem cells; however, the molecular mechanisms that contribute to generation of these germ stem cells have not been thoroughly investigated.

Results

In our studies, Overexpression of the Notch1 NICD promoted development of the reproductive ridge, but inhibited the formation of seminiferous tubules. The formation efficiency of PGCs in the reproductive ridge following overexpression of NICD (7.5% ± 0.11) was significantly higher than that (4.9% ± 0.17, p < 0.05) following inhibition of NICD, While the formation efficiency of spermatogonial stem cells (SSCs) in the testes (12.7% ± 0.08) was significantly lower after NICD overexpression than that after inhibition of NICD (16.3% ± 0.

SUBMITTER: Zuo Q 

PROVIDER: S-EPMC6009047 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature