Evolution and Diversity of Pre-mRNA Splicing in Highly Reduced Nucleomorph Genomes.

ABSTRACT: Eukaryotic genes are interrupted by introns that are removed in a conserved process known as pre-mRNA splicing. Though well-studied in select model organisms, we are only beginning to understand the variation and diversity of this process across the tree of eukaryotes. We explored pre-mRNA splicing and other features of transcription in nucleomorphs, the highly reduced remnant nuclei of secondary endosymbionts. Strand-specific transcriptomes were sequenced from the cryptophyte Guillardia theta and the chlorarachniophyte Bigelowiella natans, whose plastids are derived from red and green algae, respectively. Both organisms exhibited elevated nucleomorph antisense transcription and gene expression relative to their respective nuclei, suggesting unique properties of gene regulation and transcr

SUBMITTER: Wong DK 

PROVIDER: S-EPMC6009652 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature