The TubR-centromere complex adopts a double-ring segrosome structure in Type III partition systems.

ABSTRACT: In prokaryotes, the centromere is a specialized segment of DNA that promotes the assembly of the segrosome upon binding of the Centromere Binding Protein (CBP). The segrosome structure exposes a specific surface for the interaction of the CBP with the motor protein that mediates DNA movement during cell division. Additionally, the CBP usually controls the transcriptional regulation of the segregation system as a cell cycle checkpoint. Correct segrosome functioning is therefore indispensable for accurate DNA segregation. Here, we combine biochemical reconstruction and structural and biophysical analysis to bring light to the architecture of the segrosome complex in Type III partition systems. We present the particular features of the centromere site, tubC, of the model system encoded in Clo

SUBMITTER: Martin-Garcia B 

PROVIDER: S-EPMC6009700 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature