Absolute quantitation of microbiota abundance in environmental samples.

ABSTRACT:

Background

Microbial communities (microbiota) influence human and animal disease and immunity, geochemical nutrient cycling and plant productivity. Specific groups, including bacteria, archaea, eukaryotes or fungi, are amplified by PCR to assess the relative abundance of sub-groups (e.g. genera). However, neither the absolute abundance of sub-groups is revealed, nor can different amplicon families (i.e. OTUs derived from a specific pair of PCR primers such as bacterial 16S, eukaryotic 18S or fungi ITS) be compared. This prevents determination of the absolute abundance of a particular group and domain-level shifts in microbiota abundance can remain undetected.

Results

We have developed absolute quantitation of amplicon families using synthetic chimeric DNA spikes. Synthetic s

SUBMITTER: Tkacz A 

PROVIDER: S-EPMC6009823 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature