Cloning, expression and purification of the α-carbonic anhydrase from the mantle of the Mediterranean mussel, Mytilus galloprovincialis.

ABSTRACT: We cloned, expressed, purified, and determined the kinetic constants of the recombinant α-carbonic anhydrase (rec-MgaCA) identified in the mantle tissue of the bivalve Mediterranean mussel, Mytilus galloprovincialis. In metazoans, the α-CA family is largely represented and plays a pivotal role in the deposition of calcium carbonate biominerals. Our results demonstrated that rec-MgaCA was a monomer with an apparent molecular weight of about 32 kDa. Moreover, the determined kinetic parameters for the CO₂ hydration reaction were k_cat =  4.2 × 10⁵ s^-1 and k_cat/K_m of 3.5 × 10⁷ M^-1 ×s^-1. Curiously, the rec-MgaCA showed a very similar kinetic and acetazolamide inhibition features when compared to th

SUBMITTER: Perfetto R 

PROVIDER: S-EPMC6010101 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature