Chemoenzymatic synthesis of sialylated lactuloses and their inhibitory effects on Staphylococcus aureus.

ABSTRACT:

Background

Sialylated glycoconjugates play important roles in physiological and pathological processes. However, available sialylated oligosaccharides source is limited which is a barrier to study their biological roles. This work reports an efficient approach to produce sialic acid-modified lactuloses and investigates their inhibitory effects on Staphylococcus aureus (S. aureus).

Methods

A one-pot two-enzyme (OPTE) sialylation system was used to efficiently synthesize sialylated lactuloses. Silica gel flash chromatography column was employed to purify the sialylated products. The purity and identity of the product structures were confirmed with mass spectrometry (MS) and nuclear magnetic resonance (NMR). The inhibitory effect of sialylated lactuloses against S. aureus was e

SUBMITTER: Zeng J 

PROVIDER: S-EPMC6010273 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature