Identification of hepatic protein-protein interaction targets for betaine homocysteine S-methyltransferase.

ABSTRACT: Protein-protein interactions are an important mechanism for the regulation of enzyme function allowing metabolite channeling, crosstalk between pathways or the introduction of post-translational modifications. Therefore, a number of high-throughput studies have been carried out to shed light on the protein networks established under different pathophysiological settings. Surprisingly, this type of information is quite limited for enzymes of intermediary metabolism such as betaine homocysteine S-methyltransferase, despite its high hepatic abundancy and its role in homocysteine metabolism. Here, we have taken advantage of two approaches, affinity purification combined with mass spectrometry and yeast two-hybrid, to further uncover the array of interactions of betaine homocysteine S-methyltra

SUBMITTER: Garrido F 

PROVIDER: S-EPMC6010280 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature