Host genotype-specific microbiota do not influence the susceptibility of D. magna to a bacterial pathogen.

ABSTRACT: Host-associated microbiota have been claimed to play a role in hosts' responses to parasitic infections, often protecting the hosts from infection. We tested for such a role in the crustacean Daphnia and the parasitic bacterium Pasteuria ramosa, a widely used model system for host-parasite interactions. We first determined the infection phenotype (i.e., resistotype) of eight clonal D. magna genotypes against four strains of P. ramosa by attachment test, followed by 16 S rDNA amplicon sequencing to determine if their genotype or their parasite resistotype influences the composition of their microbiome. We then reciprocally transplanted the microbiota of two host genotypes with opposite resistotypes to four P. ramosa isolates, followed by a reassessment of their resistotype after transplanta

SUBMITTER: Sison-Mangus MP 

PROVIDER: S-EPMC6010447 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature