Detection of analytes in mitochondria without interference from other sites based on an innovative ratiometric fluorophore.

ABSTRACT: Mitochondria are vital organelles that not only produce cellular energy but also participate in many biological processes. Recently, various fluorescent probes have been developed for mitochondrial imaging. However, due to the lack of suitable dyes or strategies, it is difficult for most reported mitochondrial targeting probes to prove whether the analytes they detected are from mitochondria. In addition, positive charge on mitochondrial probes can seriously affect the mitochondrial environment. To address these issues, we herein put forward a novel strategy for probe design based on a smart NIR dye (HDFL) for mitochondrial targeting detection. Compared to general mitochondrial targeting probes that are modified with a target site and a reaction site, the new strategy is to combine

SUBMITTER: Ren TB 

PROVIDER: S-EPMC6011035 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature