Immunosuppressive Treg cells acquire the phenotype of effector-T cells in chronic lymphocytic leukemia patients.

ABSTRACT:

Background

In chronic lymphocytic leukemia (CLL) disease onset and progression are influenced by the behavior of specific CD4⁺ T cell subsets, such as T regulatory cells (Tregs). Here, we focused on the phenotypic and functional characterization of Tregs in CLL patients to improve our understanding of the putative mechanism by which these cells combine immunosuppressive and effector-like properties.

Methods

Peripheral blood mononuclear cells were isolated from newly diagnosed CLL patients (n = 25) and healthy volunteers (n = 25). The phenotypic and functional characterization of Tregs and their subsets was assessed by flow cytometry. In vitro analysis of TH1, TH2, TH17 and Tregs cytokines was evaluated by IFN-γ, IL-4, IL-17A and IL-10 secretion assays. The transcr

SUBMITTER: De Matteis S 

PROVIDER: S-EPMC6011245 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature