Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique.

ABSTRACT:

Background

Ralstonia eutropha is an important bacterium for the study of polyhydroxyalkanoates (PHAs) synthesis and CO₂ fixation, which makes it a potential strain for industrial PHA production and attractive host for CO₂ conversion. Although the bacterium is not recalcitrant to genetic manipulation, current methods for genome editing based on group II introns or single crossover integration of a suicide plasmid are inefficient and time-consuming, which limits the genetic engineering of this organism. Thus, developing an efficient and convenient method for R. eutropha genome editing is imperative.

Results

An efficient genome editing method for R. eutropha was developed using an electroporation-based CRISPR-Cas9 technique. In our stu

SUBMITTER: Xiong B 

PROVIDER: S-EPMC6011247 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature