WDR5 supports colon cancer cells by promoting methylation of H3K4 and suppressing DNA damage.

ABSTRACT:

Background

KMT2/MLL proteins are commonly overexpressed or mutated in cancer and have been shown to support cancer maintenance. These proteins are responsible for methylating histone 3 at lysine 4 and promoting transcription and DNA synthesis; however, they are inactive outside of a multi-protein complex that requires WDR5. WDR5 has been implicated in cancer for its role in the COMPASS complex and its interaction with Myc; however, the role of WDR5 in colon cancer has not yet been elucidated.

Methods

WDR5 expression was evaluated using RT-qPCR and western blot analysis. Cell viability and colony forming assays were utilized to evaluate the effects of WDR5 depletion or inhibition in colon cancer cells. Downstream effects of WDR5 depletion and inhibition were observed by weste

SUBMITTER: Neilsen BK 

PROVIDER: S-EPMC6011590 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature