Transcriptome analysis and anaerobic C₄ -dicarboxylate transport in Actinobacillus succinogenes.

ABSTRACT: A global transcriptome analysis of the natural succinate producer Actinobacillus succinogenes revealed that 353 genes were differentially expressed when grown on various carbon and energy sources, which were categorized into six functional groups. We then analyzed the expression pattern of 37 potential C₄ -dicarboxylate transporters in detail. A total of six transporters were considered potential fumarate transporters: three transporters, Asuc_1999 (Dcu), Asuc_0304 (DASS), and Asuc_0270-0273 (TRAP), were constitutively expressed, whereas three others, Asuc_1568 (DASS), Asuc_1482 (DASS), and Asuc_0142 (Dcu), were differentially expressed during growth on fumarate. Transport assays under anaerobic conditions with [¹⁴ C]fumarate and [¹⁴ C]succinate were perfor

SUBMITTER: Rhie MN 

PROVIDER: S-EPMC6011838 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature