Validation of biofilm formation on human skin wound models and demonstration of clinically translatable bacteria-specific volatile signatures.

ABSTRACT: Biofilms are major contributors to delayed wound healing and there is a need for clinically relevant experimental models to assess theranostics. Microorganisms release volatile organic compounds (VOCs) and the ability to identify these in infected cutaneous wounds could lead to efficient non-invasive diagnosis. The aims here were to develop and assess bacterial biofilm formation and identify their VOC profiles in an in vitro model and validate in human ex vivo incisional and excisional cutaneous wound models. Biofilm development was assessed using multiple microscopy techniques with biofilm-forming deficient controls and quantified using metabolic and biomass assays; and VOC production measured by gas chromatography-mass spectrometry. The production of most VOCs was affected by biofilm dev

SUBMITTER: Ashrafi M 

PROVIDER: S-EPMC6013498 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature