Delivering the cell-impermeable DNA 'light-switching' Ru(ii) complexes preferentially into live-cell nucleus via an unprecedented ion-pairing method.

ABSTRACT: The dipyridophenazine (dppz) based ruthenium polypyridyl complexes are known as molecular 'light-switches' for DNA. This property is poised to serve in diagnostic and therapeutic applications, but the poor cellular uptake restricts their use in live cells. Herein, we show that the cellular uptake, and more interestingly and surprisingly, the nuclear uptake of cell-impermeable Ru(ii)-polypyridyl cationic complexes such as [Ru(bpy)₂(dppz)]²⁺ were remarkably enhanced by three structurally unrelated biochemical agents (pentachlorophenol, carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone and tolfenamic acid), by forming lipophilic and relatively stable ion-pair complexes, via a passive diffusion mechanism. Enantioselective imaging of live-cell nuclear DNA

SUBMITTER: Zhu BZ 

PROVIDER: S-EPMC6013783 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature