Structure determination of microbial metabolites by the crystalline sponge method.

ABSTRACT: The structures of metabolites produced in microgram quantities by enzymatic reductions with baker's yeast were analyzed using the crystalline sponge method. The X-ray data provided reliable structures for trace metabolites including their relative and absolute stereochemistries that are not fully addressed by conventional NMR and LC-MS analyses. Technically, combining two or more chromatographic purification techniques is essential because, unlike abundant synthetic compounds, extracted metabolites contain many low level UV-silent impurities. The crystalline sponge method coupled with HPLC purification (LC-SCD) would thus be a useful method for metabolic analysis and drug discovery.

SUBMITTER: Inokuma Y 

PROVIDER: S-EPMC6013795 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature