Chronic 2P-STED imaging reveals high turnover of dendritic spines in the hippocampus in vivo.

ABSTRACT: Rewiring neural circuits by the formation and elimination of synapses is thought to be a key cellular mechanism of learning and memory in the mammalian brain. Dendritic spines are the postsynaptic structural component of excitatory synapses, and their experience-dependent plasticity has been extensively studied in mouse superficial cortex using two-photon microscopy in vivo. By contrast, very little is known about spine plasticity in the hippocampus, which is the archetypical memory center of the brain, mostly because it is difficult to visualize dendritic spines in this deeply embedded structure with sufficient spatial resolution. We developed chronic 2P-STED microscopy in mouse hippocampus, using a 'hippocampal window' based on resection of cortical tissue and a long working distance obj

SUBMITTER: Pfeiffer T 

PROVIDER: S-EPMC6014725 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature