Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow.

ABSTRACT: Endothelial cells (ECs) contribute to haematopoietic stem cell (HSC) maintenance in bone marrow, but the differential contributions of EC subtypes remain unknown, owing to the lack of methods to separate with high purity arterial endothelial cells (AECs) from sinusoidal endothelial cells (SECs). Here we show that the combination of podoplanin (PDPN) and Sca-1 expression distinguishes AECs (CD45^- Ter119^- Sca-1^bright PDPN^-) from SECs (CD45^- Ter119^- Sca-1^dim PDPN⁺). PDPN can be substituted for antibodies against the adhesion molecules ICAM1 or E-selectin. Unexpectedly, prospective isolation reveals that AECs secrete nearly all detectable EC-derived stem cell factors (SCF). Genetic deletion of Scf in AECs,

SUBMITTER: Xu C 

PROVIDER: S-EPMC6015052 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature