[Expression of transient receptor potential canonical channel proteins in human non-small cell lung cancer].

ABSTRACT:

Background and objective

Transient receptor potential canonical (TRPC) proteins, a group of Ca2' permeable nonselective cation channels, are thought to constitute store-operated calcium channels (SOCC) and mediate store-operated calcium entry (SOCE) in various cell types. Members of TRPC have been found to be involved in abnormal proliferation, differentiation, and growth of cancer cells. The aim of this study is to detect the mRNA and protein expression of TRPC in non-small cell lung cancer (NSCLC).

Methods

Real-time quantitative PCRwas performed to screen the expression of TRPC mRNA in NSCLC tissue. Protein expression of TRPC was detected by Western blot.

Results

Among the seven family members of TRPC so far identified (TRPC1-7), we detected the expression ofTRPC1,

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC6015152 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature