A ¹³C isotope labeling method for the measurement of lignin metabolic flux in Arabidopsis stems.

ABSTRACT:

Background

Metabolic fluxes represent the functional phenotypes of biochemical pathways and are essential to reveal the distribution of precursors among metabolic networks. Although analysis of metabolic fluxes, facilitated by stable isotope labeling and mass spectrometry detection, has been applied in the studies of plant metabolism, we lack experimental measurements for carbon flux towards lignin, one of the most abundant polymers in nature.

Results

We developed a feeding strategy of excised Arabidopsis stems with ¹³C labeled phenylalanine (Phe) for the analysis of lignin biosynthetic flux. We optimized the feeding methods and found the stems continued to grow and lignify. Consistent with lignification profiles along the stems, higher levels of phenylpropanoids

SUBMITTER: Wang P 

PROVIDER: S-EPMC6015466 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature