Knockdown of Chloride Channel-3 Inhibits Breast Cancer Growth In Vitro and In Vivo.

ABSTRACT:

Purpose

Chloride channel-3 (ClC-3) is a member of the chloride channel family and plays a critical role in a variety of cellular activities. The aim of the present study is to explore the molecular mechanisms underlying the antitumor effect of silencing ClC-3 in breast cancer.

Methods

Human breast cancer cell lines MDA-MB-231 and MCF-7 were used in the experiments. Messenger RNA and protein expression were examined by quantitative real-time polymerase chain reaction and western blot analysis. Cell proliferation was measured by the bromodeoxyuridine method, and the cell cycle was evaluated using fluorescence-activated cell sorting. Protein interaction in cells was analyzed by co-immunoprecipitation. Tumor tissues were stained with hematoxylin-eosin and tumor burden was measur

SUBMITTER: Zhou FM 

PROVIDER: S-EPMC6015970 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature