Stepwise metabolic engineering of Escherichia coli to produce triacylglycerol rich in medium-chain fatty acids.

ABSTRACT:

Background

Triacylglycerols (TAGs) rich in medium-chain fatty acids (MCFAs, C10-14 fatty acids) are valuable feedstocks for biofuels and chemicals. Natural sources of TAGs rich in MCFAs are restricted to a limited number of plant species, which are unsuitable for mass agronomic production. Instead, the modification of seed or non-seed tissue oils to increase MCFA content has been investigated. In addition, microbial oils are considered as promising sustainable feedstocks for providing TAGs, although little has been done to tailor the fatty acids in microbial TAGs.

Results

Here, we first assessed various wax synthase/acyl-coenzyme A:diacylglycerol acyltransferases, phosphatidic acid phosphatases, acyl-CoA synthetases as well as putative fatty acid metabolism regulators for pr

SUBMITTER: Xu L 

PROVIDER: S-EPMC6016142 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature