TRIM44, a crucial target of miR-410, functions as a potential oncogene in osteosarcoma.

ABSTRACT:

Purpose

Mounting evidence highlights the essential role of TRIM44 in tumor initiation and malignant progression in several cancers; however, the function of TRIM44 in osteosarcoma (OS) remains unknown. In this study, we aim to investigate the role of TRIM44 and reveal its regulation by deregulated miRNAs in OS.

Materials and methods

The expression profiles of TRIM44 were examined by immunohistochemistry, Western blotting, and qRT-PCR. The biological functions of TRIM44 were investigated through siRNA-mediated knockdown experiments. The regulation of TRIM44 by miR-410 was confirmed by Western blotting, dual luciferase reporter assays, and rescue experiments.

Results

TRIM44 was upregulated in OS tissues and cell lines, an

SUBMITTER: Wang H 

PROVIDER: S-EPMC6016597 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature