Synthetic bottom-up approach reveals the complex interplay of Shigella effectors in regulation of epithelial cell death.

ABSTRACT: Over the course of an infection, many Gram-negative bacterial pathogens use complex nanomachines to directly inject tens to hundreds of proteins (effectors) into the cytosol of infected host cells. These effectors rewire processes to promote bacterial replication and spread. The roles of effectors in pathogenesis have traditionally been investigated by screening for phenotypes associated with their absence, a top-down approach that can be limited, as effectors often act in a functionally redundant or additive manner. Here we describe a synthetic Escherichia coli-based bottom-up platform to conduct gain-of-function screens for roles of individual Shigella effectors in pathogenesis. As proof of concept, we screened for Shigella effectors that limit cell death induced on

SUBMITTER: Mou X 

PROVIDER: S-EPMC6016774 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature