Uncovering pH at both sides of the root plasma membrane interface using noninvasive imaging.

ABSTRACT: Building a proton gradient across a biological membrane and between different tissues is a matter of great importance for plant development and nutrition. To gain a better understanding of proton distribution in the plant root apoplast as well as across the plasma membrane, we generated Arabidopsis plants expressing stable membrane-anchored ratiometric fluorescent sensors based on pHluorin. These sensors enabled noninvasive pH-specific measurements in mature root cells from the medium-epidermis interface up to the inner cell layers that lie beyond the Casparian strip. The membrane-associated apoplastic pH was much more alkaline than the overall apoplastic space pH. Proton concentration associated with the plasma membrane was very stable, even when the growth medium pH was altered. T

SUBMITTER: Martiniere A 

PROVIDER: S-EPMC6016826 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature