Assessment of HIF-1α expression and release following endothelial injury in-vitro and in-vivo.

ABSTRACT:

Background

Endothelial injury is an early and enduring feature of cardiovascular disease. Inflammation and hypoxia may be responsible for this, and are often associated with the up-regulation of several transcriptional factors that include Hypoxia Inducible Factor-1 (HIF-1). Although it has been reported that HIF-1α is detectable in plasma, it is known to be unstable. Our aim was to optimize an assay for HIF-1α to be applied to in vitro and in vivo applications, and to use this assay to assess the release kinetics of HIF-1α following endothelial injury.

Methods

An ELISA for the measurement of HIF-1α in cell-culture medium and plasma was optimized, and the assay was used to determine the best conditions for sample collection and storage. The results of the ELISA were validate

SUBMITTER: Heikal L 

PROVIDER: S-EPMC6016879 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature