An optimized methodology for whole genome sequencing of RNA respiratory viruses from nasopharyngeal aspirates.

ABSTRACT: Over the last decade, the number of viral genome sequences deposited in available databases has grown exponentially. However, sequencing methodology vary widely and many published works have relied on viral enrichment by viral culture or nucleic acid amplification with specific primers rather than through unbiased techniques such as metagenomics. The genome of RNA viruses is highly variable and these enrichment methodologies may be difficult to achieve or may bias the results. In order to obtain genomic sequences of human respiratory syncytial virus (HRSV) from positive nasopharyngeal aspirates diverse methodologies were evaluated and compared. A total of 29 nearly complete and complete viral genomes were obtained. The best performance was achieved with a DNase I treatment to the RNA direc

SUBMITTER: Goya S 

PROVIDER: S-EPMC6016902 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature