VpStyA1/VpStyA2B of Variovorax paradoxus EPS: An Aryl Alkyl Sulfoxidase Rather than a Styrene Epoxidizing Monooxygenase.

ABSTRACT: Herein we describe the first representative of an E2-type two-component styrene monooxygenase of proteobacteria. It comprises a single epoxidase protein (VpStyA1) and a two domain protein (VpStyA2B) harboring an epoxidase (A2) and a FAD-reductase (B) domain. It was annotated as VpStyA1/VpStyA2B of Variovorax paradoxus EPS. VpStyA2B serves mainly as NADH:FAD-oxidoreductase. A K_m of 33.6 ± 4.0 µM for FAD and a k_cat of 22.3 ± 1.1 s^-1 were determined and resulted in a catalytic efficiency (k_catK_m^-1) of 0.64 s^-1 μM^-1. To investigate its NADH:FAD-oxidoreductase function the linker between A2- and B-domain (AREAV) was mutated. One mutant (AA

SUBMITTER: Tischler D 

PROVIDER: S-EPMC6017014 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature