Xylosylated Detoxification of the Rice Flavonoid Phytoalexin Sakuranetin by the Rice Sheath Blight Fungus Rhizoctonia solani.

ABSTRACT: Sakuranetin (1) is a rice flavanone-type phytoalexin. We have already reported that the metabolites from the detoxification of 1 by Pyriculariaoryzae are naringenin (2) and sternbin. In this study, we investigated whether the rice sheath blight fungus Rhizoctoniasolani, another major rice pathogen, can detoxify 1. The extract of R. solani suspension culture containing 1 was analyzed by LC-MS to identify the metabolites of 1. Three putative metabolites of 1 were detected in the extract from the R. solani suspension culture 12 h after the addition of 1, and they were identified as 2, sakuranetin-4'-O-β-d-xylopyranoside (3), and naringenin-7-O-β-d-xylopyranoside (4) by NMR, LC-MS/MS, and GC-MS analyses. The accumulation of 2, 3, and 4 reached their maximum levels 9-12 h after the addition of

SUBMITTER: Katsumata S 

PROVIDER: S-EPMC6017487 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature