Analytical Approaches to Improve Accuracy in Solving the Protein Topology Problem.

ABSTRACT: To take advantage of recent advances in genomics and proteomics it is critical that the three-dimensional physical structure of biological macromolecules be determined. Cryo-Electron Microscopy (cryo-EM) is a promising and improving method for obtaining this data, however resolution is often not sufficient to directly determine the atomic scale structure. Despite this, information for secondary structure locations is detectable. De novo modeling is a computational approach to modeling these macromolecular structures based on cryo-EM derived data. During de novo modeling a mapping between detected secondary structures and the underlying amino acid sequence must be identified. DP-TOSS (Dynamic Programming for determining the Topology Of Secondary Structures) is one tool that attempts to auto

SUBMITTER: Al Nasr K 

PROVIDER: S-EPMC6017786 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature