Co-Localization of Crotamine with Internal Membranes and Accentuated Accumulation in Tumor Cells.

ABSTRACT: Crotamine is a highly cationic; cysteine rich, cross-linked, low molecular mass cell penetrating peptide (CPP) from the venom of the South American rattlesnake. Potential application of crotamine in biomedicine may require its large-scale purification. To overcome difficulties related with the purification of natural crotamine (nCrot) we aimed in the present study to synthesize and characterize a crotamine analog (sCrot) as well investigate its CPP activity. Mass spectrometry analysis demonstrates that sCrot and nCrot have equal molecular mass and biological function—the capacity to induce spastic paralysis in the hind limbs in mice. sCrot CPP activity was evaluated in a wide range of tumor and non-tumor cell tests performed at different time points. We demonstrate that sCrot-Cy3 showed di

SUBMITTER: Mambelli-Lisboa NC 

PROVIDER: S-EPMC6017820 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature