Spatiotemporal endothelial cell - pericyte association in tumors as shown by high resolution 4D intravital imaging.

ABSTRACT: Endothelial cells and pericytes are integral cellular components of the vasculature with distinct interactive functionalities. To study dynamic interactions between these two cells we created two transgenic animal lines. A truncated eNOS (endothelial nitric oxide synthase) construct was used as a GFP tag for endothelial cell evaluation and an inducible Cre-lox recombination, under control of the Pdgfrb (platelet derived growth factor receptor beta) promoter, was created for pericyte assessment. Also, eNOStag-GFP animals were crossed with the already established Cspg4-DsRed mice expressing DsRed fluorescent protein in pericytes. For intravital imaging we used tumors implanted in the dorsal skinfold of these transgenic animals. This setup allowed us to study time and space dependent complexi

SUBMITTER: Seynhaeve ALB 

PROVIDER: S-EPMC6018425 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature