Loss of Glis3 causes dysregulation of retrotransposon silencing and germ cell demise in fetal mouse testis.

ABSTRACT: Fetal germ cell development is regulated by an elaborate combination of cell-extrinsic and cell-intrinsic signals. Here we identify a novel role for the Krüppel-like transcription factor Gli-Similar 3 (Glis3) in male germ cell development in the mouse embryos. Glis3 is expressed in male germ cells during the brief window of time prior to initiation of piRNA-dependent retrotransposon surveillance. Disruption of Glis3 function led to a widespread reduction in retrotransposon silencing factors, aberrant retrotransposon expression and pronounced germ cell loss. Experimental induction of precocious Glis3 expression in vivo before its normal expression resulted in premature expression of several piRNA pathway members, suggesting that GLIS3 is necessary for the activation of the retrotransposon s

SUBMITTER: Ungewitter EK 

PROVIDER: S-EPMC6018429 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature