Comparison of multi-lineage differentiation of hiPSCs reveals novel miRNAs that regulate lineage specification.

ABSTRACT: MicroRNAs (miRNAs) are known to be crucial players in governing the differentiation of human induced pluripotent stem cells (hiPSCs). Despite their utter importance, identifying key lineage specifiers among the myriads of expressed miRNAs remains challenging. We believe that the current practice in mining miRNA specifiers via delineating dynamic fold-changes only is inadequate. Our study, therefore, provides evidence to pronounce "lineage specificity" as another important attribute to qualify for these lineage specifiers. Adopted hiPSCs were differentiated into representative lineages (hepatic, nephric and neuronal) over all three germ layers whilst the depicted miRNA expression changes compiled into an integrated atlas. We demonstrated inter-lineage analysis shall aid in the identificatio

SUBMITTER: Li L 

PROVIDER: S-EPMC6018499 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature