Aberration correction considering curved sample surface shape for non-contact two-photon excitation microscopy with spatial light modulator.

ABSTRACT: In this paper, excitation light wavefront modulation is performed considering the curved sample surface shape to demonstrate high-quality deep observation using two-photon excitation microscopy (TPM) with a dry objective lens. A large spherical aberration typically occurs when the refractive index (RI) interface between air and the sample is a plane perpendicular to the optical axis. Moreover, the curved sample surface shape and the RI mismatch cause various aberrations, including spherical ones. Consequently, the fluorescence intensity and resolution of the obtained image are degraded in the deep regions. To improve them, we designed a pre-distortion wavefront for correcting the aberration caused by the curved sample surface shape by using a novel, simple optical path length difference ca

SUBMITTER: Matsumoto N 

PROVIDER: S-EPMC6018692 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature