The Role of NRG1 in the Predisposition to Papillary Thyroid Carcinoma.

ABSTRACT:

Context

Previous genome-wide association studies have shown that single-nucleotide polymorphism (SNP) rs2439302 in chromosome 8p12 is significantly associated with papillary thyroid carcinoma (PTC) risk and dysregulated NRG1 expression. The underlying mechanisms remain to be discovered.

Objective

To evaluate the expression of NRG1 isoforms, candidate functional variants, and potential genes downstream of NRG1 in thyroid tissue.

Methods

Quantitative reverse transcription polymerase chain reaction was applied for gene expression analysis. SNaPshot assay, haplotype, and computer analyses were performed to evaluate candidate functional variants. Other functional assays [chromatin immunoprecipitation (ChIP) assay, luciferase assay, small interfering RNA knockdown, and RNA

SUBMITTER: He H 

PROVIDER: S-EPMC6018707 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature