Characterization of migratory primordial germ cells in the aorta-gonad-mesonephros of a 4.5-week-old human embryo: a toolbox to evaluate in vitro early gametogenesis.

ABSTRACT:

Study question

Which set of antibodies can be used to identify migratory and early post-migratory human primordial germ cells (hPGCs)?

Study finding

We validated the specificity of 33 antibodies for 31 markers, including POU5F1, NANOG, PRDM1 and TFAP2C as specific markers of hPGCs at 4.5 weeks of development of Carnegie stage (CS12-13), whereas KIT and SOX17 also marked the intra-aortic hematopoietic stem cell cluster in the aorta-gonad-mesonephros (AGM).

What is known already

The dynamics of gene expression during germ cell development in mice is well characterized and this knowledge has proved crucial to allow the development of protocols for the in vitro derivation of functional gametes. Although there is a great interest in generating human gametes in vitro, it is

SUBMITTER: Gomes Fernandes M 

PROVIDER: S-EPMC6018722 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature