Influence of the viscosity of healthy and diseased human mucins on the motility of Helicobacter pylori.

ABSTRACT: We present particle tracking microrheology results on human mucins, isolated from normal surface and gland mucosa and one tumor sample, and examine the motility of Helicobacter pylori in these mucins. At 1.5% concentration human mucin solutions are purely viscous, with viscosity η (gland mucin) > η (surface mucin) > η (tumor mucin). In the presence of motile H. pylori bacteria, particle diffusion is enhanced, with diffusivity D_+bac(tumor mucin) > D_+bac(gland mucin) > D_+bac(surface mucin). The surface and tumor mucin solutions exhibit an elastic response in the presence of bacteria. Taken together these results imply that particle diffusion and active swimming are coupled and impact the rheology of mucin solutions. Both J99 wild type (WT) and its isogenic Δb

SUBMITTER: Su C 

PROVIDER: S-EPMC6018794 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature